Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 2, February 2008, pp. 144-149
Simultaneous HPLC Determination of Caffeine,
Theobromine, and Theophylline in Food, Drinks, and Herbal Products
Branislava Srdjenovic[1], Vukosava Djordjevic-Milic[1],
Nevena Grujic[1], Rade Injac[2], and Zika Lepojevic[3]
[1]Medical Faculty, Department of Pharmacy, University of Novi
Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia;
[2]Faculty of
Pharmacy, Department of Pharmaceutical Biology, University of
Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia; and
[3]Faculty
of Technology, University of Novi Sad, Cara Lazara 1, 21000 Novi
Sad, Serbia
A rapid and selective high-performance liquid
chromatographic (HPLC) method is developed for the separation
and determination of caffeine, theobromine, and theophylline.
The chromatography is performed on a Zorbax Eclipse XDB-C8 column
(4.6 ¥ 150 mm i.d., 5-µm particle size) at 25°C,
with a mobile phase of water–THF (0.1% THF in water, pH
8)–acetonitrile (90:10, v/v). The flow rate is 0.8 mL/min,
and detection is by UV at 273 nm. This method permits the simultaneous
determination of caffeine, theobromine, and theophylline in food,
drinks, and herbal products with detection limits of 0.07–0.2
mg/L and recoveries of 100.20–100.42%. Correlation coefficients,
for the calibration curves in the linear range of 0.2–100
mg/L, are greater than 0.9999 for all compounds. The within-
and between-day precision is determined for both retention times
and peak area. The data suggests that the proposed HPLC method
can be used for routine quality control of food, drinks, and
herbal products.
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