Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 2, February 2008, pp. 137-143
Determination of Caffeine and Associated Compounds
in Food, Beverages, Natural Products, Pharmaceuticals, and Cosmetics
by Micellar Electrokinetic Capillary Chromatography
Rade Injac[1], Branislava Srdjenovic[2], Matevz
Prijatelj[3], Marija Boskovic[4], Katarina Karljikovic-Rajic[5],
and Borut Strukelj[1]
[1]Faculty of Pharmacy, Department of Pharmaceutical Biology,
University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia;
[2]Medical Faculty, Department of Pharmacy, University of Novi
Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia;
[3]Faculty of
Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana,
Slovenia;
[4]Faculty of Pharmacy, Department of Pharmakocinetics and Biopharmacetics,
University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia;
and
[5]Faculty of Pharmacy, Institute of Analytical Chemistry,
University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia
A method is described for quantitating caffeine,
theobromine, theophylline, paracetamol, propyphenazone, acetylsalicylic
acid, salicylic acid, and codeine phosphate in corresponding
real samples of food, beverages, natural products, pharmaceuticals,
and cosmetic preparations by micellar electrokinetic capillary
chromatography. The separation is carried out at 25°C and
25 kV, using a 20mM phosphate buffer (pH 9.0), 80mM sodium dodecyl
sulfate, and 7.5% (v/v) acetonitrile. UV detection is at 210
nm. The method is shown to be specific, accurate (recoveries
over the range 98.9–101.2%), linear over the tested range
(correlation coefficients ≥ 0.9993), and precise (relative
standard deviation below 2.1%). The method is applied for the
quantitative analysis of these compounds in different foods,
beverages, natural products, pharmaceuticals, and cosmetic products.
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