Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 2, February 2008, pp. 122-126
The Rapid Determination of Artemisinin by Post-Column
Derivatization High-Performance Liquid Chromatography Using Matrix
Solid-Phase Dispersion Method
Hongcheng Liu[1], Qiwan Li[1], Shaoping Li[2],
Yanhong Zou[1], and Anyun Gu[2]
[1]Kunming Supervision and Testing Center for Farm Product Quality,
Ministry of Agriculture, (Kunming), Yunnan Academy of Agriculture
Science, Quality Standard and Testing Technology Institute, Kunming,
650223, China and
[2]Medicinal Plants Research Institute, Kunming,
650223, China
Artemisinin (an antimalaric compound) is isolated
as the active compound of the medicinal plant Artemisia annua
L. A simple, rapid, and high-efficient method of extraction is
developed, in which it is extracted by matrix solid-phase dispersion
(MSPD) and directly analyzed by post-column derivatization high-performance
liquid chromatography (HPLC). The quantitation results from the
MSPD method are compared with two conventional liquid solvent
extraction processes, Soxhlet and ultrasonic wave by F-test,
and the result indicates no significant difference. The production
rate of Artemisinin during wild plant growth (tested over two
years, acquired from Yunnan, provincial Yuan Yan country) is
determined. The recovery range of determination is 88.1–91.2%.
The relative standard deviation (RSD) is 4.55–6.43%. The
limits of detection are 0.1 µg/mL, and the limits of quantitation
are 0.5 µg/mL.
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