Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 2, February 2008, pp. 117-121
Rapid Microwave-Assisted Hydrolysis for Determination
of Ginkgo Flavonol Glycosides in Extracts of Ginkgo biloba Leaves
J. Xiping Chen[1,2] and Yangde Zhang[1],
[1]National Hepatobiliary & Enteric Surgery Research Center,
Ministry of Health, Changsha 410008, China and
[2]Hunan Traditional
Chinese Medicine College, Changsha 410007, China
A rapid microwave-assisted hydrolysis (MAH) method
is presented for the sample pretreatment of the determination
of ginkgo flavonol glycosides in extracts of Ginkgo biloba L.
(EGb). By this method, flavonol glycosides can be completely
hydrolyzed within 2 min. After investigating the effects of solvents,
acidity, microwave power, and microwave radiation time on hydrolysis,
the optimal hydrolysis conditions are as follows: 300 W of microwave
power, 2 min of hydrolysis time, 5.7% of hydrochloric acid in
the hydrolysis solution, and n-propanol as the hydrolysis solvent.
After MAH of the samples, three flavonol aglycones, such as quercetin,
kaempferol, and isorhamnetin are analyzed by high-performance
liquid chromatography. Compared with conventional reflux hydrolysis,
this method owns offers several advantages: it saves time, costs
less, and is environmentally friendly.
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