Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 2, February 2008, pp. 111-116
Preparative-Scale Chromatography of Ecdysteroids:
A Class of Biologically Active Steroids
Noemi Toth and Maria Bathori
Department of Pharmacognosy, University of Szeged, H-6720 Szeged,
Eotovs u. 6, Hungary
A simple separation procedure is developed for
the isolation of the main phytoecdysteroid 20-hydroxyecdysone
from the herb Silene viridiflora. The purification in four steps
uses only a simple preparative-scale separation technique (i.e.,
liquid–liquid extraction, precipitation, solid-phase extraction
on octadecyl silica, and crystallization). This procedure is
extended using classical normal-phase liquid column chromatography,
rotation planar chromatography, and preparative high-performance
liquid chromatography for the isolation of the minor ecdysteroids:
integristerone A, 26-hydroxypolypodine B, 2-deoxy-20,26-dihydroxyecdysone,
and polypodine B. 2-Deoxy-20,26-dihydroxyecdysone is isolated
from this species for the first time. The isolation of these
ecdysteroids in adequate amounts makes them readily available
for insect physiology experiments and for structure-activity
relationship studies. The preparative-scale separation work also
results in a minor, as yet unknown ecdysteroid.
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