Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 2, February 2008, pp. 097-101
Quantitative and Qualitative Investigation of
the Main Flavonoids in Heartsease (Viola tricolor L.)
V. Vukics[1], B. Hevesi Toth[1], T. Ringer[2],
K. Ludanyi[3], A. Kery[1], G.K. Bonn[2], and A. Guttman[4],
[1]Department of Pharmacognosy, Semmelweis University, Budapest,
Hungary;
[2]Institute of Analytical Chemistry and Radiochemistry,
University of Innsbruck, Austria;
[3]Department of Pharmaceutics,
Semmelweis University, Budapest, Hungary; and
[4]Horváth
Laboratory of Bioseparation Sciences, University of Innsbruck,
Austria
Liquid chromatography coupled to electrospray
ionization tandem mass spectrometry (MSn) is used for the analysis
of flavonoids in heartsease (Viola tricolor L.). Our data suggested
that the two main flavonoid components were violanthin (6-C-glucosyl-8-C-rhamnosyl
apigenin) and rutin (3-O-rutinosyl quercetin). The identification
of rutin was confirmed by comparing its retention time, UV spectrum,
molecular mass, and fragmentation pattern with the reference
standard. In this paper, we also report on the quantitative analysis
of rutin by high-performance liquid chromatography. According
to our results, heartsease herb contained 420 ± 1.17 µg/g
rutin.
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