Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 7, August 2008, pp. 659-663
Gas Chromatographic–Mass Spectrometric
Analysis of Acrylamide and Acetamide in Cigarette Mainstream
Smoke after On-Column Injection
Joerg Diekmann1, Arno Wittig1,
and Regina Stabbert2 1Philip Morris Research Laboratories GmbH, Fuggerstr. 3, D-51149
Köln, Germany and 2Philip Morris Products S.A., PMI Research & Development,
Quai Jeanrenaud 56, CH-2000 Neuchâtel, Switzerland
A method is described for the simultaneous determination
of two short-chained amides, acrylamide and acetamide (classified
by the International Agency for Research on Cancer as probable
and possible human carcinogens, respectively), in total particulate
matter using gas chromatography-on-column injection and mass
spectrometric detection. Sample preparation is kept to a minimum,
and the proposed analytical procedure proves to be fast, sensitive,
and precise. Validation studies show good linearity with a regression
coefficient of r2 =1.000 for both compounds. Quantitation
limits are 32 ng/mL for acrylamide and 70 ng/mL for acetamide.
In the particulate phase of mainstream smoke from the University
of Kentucky Reference Cigarette 2R4F, 2.3 µg/cig acrylamide
and 4.7 µg/cig acetamide are found; no acetamide and only
.0074 µg/cig acrylamide is found in the gas phase. Possible
mechanisms of formation in cigarette smoke are discussed.
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