Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 4, April 2008, pp. 345-350
Simultaneous Determination of Five Fat-Soluble
Vitamins in Feed by High-Performance Liquid Chromatography Following
Solid-Phase Extraction
Xiuping Xue, Jinming You, and Pingli He
College of Animal Science and Technology, China Agricultural
University, No. 2 Yuanmingyuan West Road, Beijing, 100094,
P.R. China
A high-performance liquid chromatography method
is developed for the simultaneous determination of menadione,
retinyl acetate, cholecalciferol, α-tocopherol, and α-tocpherol
acetate in feed. The present study uses an enzyme to destroy
the coating film, ethanol to extract free vitamins, and Oasis
HLB cartridges to purify. Vitamins are separated using an Atlantis
dC18 column. The mobile phase is methanol–water (98:2 v/v).
Detection is performed with a UV–vis detector at 230 and
265 nm. The linearity, accuracy, and repeatability of this method
are all satisfactory. Application of the method is suitable for
the determination of the fat-soluble vitamins in general feed.
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