Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 4, April 2008, pp. 320-324
Separation and Quantitation of Jasmonic Acid
Using HPTLC
Pinakin C. Dhandhukia and Vasudev R. Thakkar
BRD School of Biosciences, Sardar Patel Maidan, Vadtal Road,
Satellite Campus, Post Box No. 39, Sardar Patel University,
Vallabh Vidyanagar-388 120 GUJARAT, INDIA
A rapid, simple, and stringent protocol for the
detection and quantitation of jasmonic acid (JA) is designed
using high-performance thin-layer chromatography. Acidified culture
filtrate of Lasiodiplodia theobromae is extracted with an equal
volume of ethyl acetate and spotted on silica gel 60 F254 foil
using Linomat-5 spray-on applicator. Standard JA is also spotted
either internally or adjacent to the sample, and the foils are
developed with isopropanol–ammonia–water
[10:1:1 (v/v)] as the mobile phase. A quantitative estimation
of the separated JA is performed by measuring the absorbance
at 295 nm in the reflective mode. The sensitivity of the method
is improved by adding internal standard to obtain a detection
limit of 1 µg. The limit of quantitation is found to be
80 µg with this method. The method is shown to have selectivity,
accuracy, precision, and high sample throughput, making it useful
for the routine analysis of JA in basic science and perfumery
industries.
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