Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 4, April 2008, pp. 304-307
HPTLC Determination of Rabeprazole and Domperidone
in Capsules and its Validation
Bhavesh H. Patel1, Bhanubhai N. Suhagia2,
Madhabhai M. Patel1, and Jignesh R. Patel1 1Department of Pharmaceutical Chemistry, S. K. Patel College
of Pharmaceutical Education and Research, Ganpat Vidyanagar,
Kherva, Mehsana-382711, Gujarat, India and
2Department of Pharmaceutical
Chemistry, L.M.College of Pharmacy, Navrangpura, Ahmedabad-380009,
Gujarat, India
This paper describes a validated high-performance
thin-layer chromatography (HPTLC) method for simultaneous estimation
of rabeprazole (RA) and domperidone (DO) in pure powder and in
capsule formulations. An HPTLC method separation is achieved
on an aluminum sheet of silica gel 60F254 using ethyl
acetate–methanol–benzene–acetonitrile
(30:20:30:20 v/v) as mobile phase. Quantitation is achieved with
UV detection at 287 nm over a concentration range of 400–1200
ng/spot and 600–1800 ng/spot with mean recovery of 99.82 ± 0.74
and 99.43 ± 0.68 for RA and DO, respectively, in the HPTLC
method. This method is simple, precise, and sensitive, and it
is applicable for the simultaneous determination of RA and DO
in pure powder and in capsule formulation.
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