Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 4, April 2008, pp. 291-297
TLC of Alkaloids on Cyanopropyl Bonded Stationary
Phases. Part II. Connection with RP18 and Silica Plates
Anna Petruczynik1, Monika Waksmundzka-Hajnos1,
Tomasz Plech1, Tomasz Tuzimski2, Michal
L. Hajnos3, Grzegorz
Jó´zwiak1, Maria Gadzikowska1,
and Anna Rompala4 1Medical University of Lublin, Department of Inorganic Chemistry;
2Medical University of Lublin, Department of Physical Chemistry;
3Medical University of Lublin, Department of Pharmacognosy; and
4Medical University of Lublin, Department of Chemistry
Some standards of the alkaloids and synthetic
or natural mixtures are separated by two-dimensional thin-layer
chromatography (TLC) on different adsorbent layers. Normal- and
reversed-phase systems are used to obtain significant differences
in the separation selectivity. Optimization of the one-dimensional
TLC separation of the alkaloids’ standards is performed
on cyanopropyl-silica, RP18W, and silica layers in various eluents
containing (besides diluent and modifier) silanol blockers, such
as diethyl amine or ammonia. The most selective systems are used
for the separation of the alkaloids’ mixtures by two-dimensional
TLC with an adsorbent gradient method. The mixtures of alkaloids
or plant extracts (Chelidonium majus, Fumaria officinalis, or
Glaucium flavum) are chromatographed in system I; the plates
are connected with the plate pre-coated with various adsorbent,
and partly separated fractions are transferred to the second
layer and developed in system II. CN-silica–RP18W and CN-silica–silica
are used as the connected layers. The alkaloids are identified
by RF values of standards, and the components of plant
extracts are identified in both systems, and by the comparison
of UV spectra obtained in diode array detector denistometry.
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