Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 8, September 2007, pp. 549-552
Quantitative and Qualitative Determination of
Liuwei Dihuang Tablets by HPLC–UV-MS–MS
Xinfeng Zhao[1], Yue Wang[1], and Yuqing Sun[2]
[1]Medical College, Dalian University, Dalian 116622, People’s
Republic of China and
[2]Department of Analytical Chemistry,
Shenyang Pharmaceutical University, Shenyang 110016, People’s
Republic of China
A method is developed for the analysis of allantoin,
gallic acid, dihydromelittoside, loganin, paeoniflorin, benzoylpaeoniflorin,
and paeonol in Liuwei Dihuang tablets by high-performance liquid
chromatography (HPLC)– UV-mass spectrometry (MS)–MS.
Gradient elution with methanol–acetonitrile–water–formic
acid solvent system is employed in the HPLC–electrospray
ionization-MS study. The positive-ion ESI mode is suitable for
these compounds. The peaks of gallic acid, loganin, dihydromelittoside,
paeoniflorin, benzoylpaeoniflorin, and paeonol are identified
by their mass spectra and the fragments of their MS–MS
spectra. Allantoin, gallic acid, loganin, paeoniflorin, and paeonol
are simultaneously determined by UV detection at 210 nm for quantitative
purposes.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00.
Visa, MasterCard & Amex accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
