Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 8, September 2007, pp. 544-548
Determination and Pharmacokinetics of 6,7-Dimethoxycoumarin
in Rat Plasma after Intragastric Administration of Different
Decoctions of Yinchenhao Tang
Zhi-guo Yu[1], Qian Wang[2], Ke Li[3], Ying-qi
Li[1], and Xiao-xia Gao[1]
[1]Department of Pharmaceutical Analysis, School of Pharmacy,
Shenyang Pharmaceutical University, Shenyang 110016, People’s
Republic of China;
[2]Shanghai Institute of Traditional Chinese
Material Medical, Shanghai 200127, People’s Republic of
China; and
[3]Laboratory of Drug Metabolism and Pharmacokinetics,
Shenyang Pharmaceutical University, Shenyang 110016, People’s
Republic of China
A simple and sensitive reversed-phase high-performance
liquid chromatography method with UV detection is developed and
validated for the determination of 6,7-dimethoxycoumarin in rat
plasma and comparative analysis of its pharmacokinetics after
intragastric administration of 6,7-dimethoxycoumarin and three
different decoctions of Yinchenhao Tang. The plasma samples are
deproteinated with acetonitrile. The components are separated
on a Kromasil C18 column (250 × 4.6 mm, 5 µm,) with
methanol–1% acetic acid solution–tetrahydrofuran
(30:63:7, v/v/v) as the mobile phase, and the UV detector is
set at 340 nm. Coumarin is used as an internal standard. The
linear calibration curve is obtained in the concentration range
of 25~2500 ng/mL. The lower limit of quantitation of the method
is 25 ng/mL. The intra- and inter-day precision are less than
12%, and the accuracy determined with relative error ranges from –2.9%
to 1.7%. The data obtained from rat plasma are analyzed with
Topfit 2.0 Pharmacokinetic Software. With pharmacokinetic analysis,
the main parameters after intragastric administration of 6,7-dimethoxycoumarin,
Herba Artemisiae Scopariae decoction, Artemisiae Scopariae decoction
plus Radix et Rhizoma Rhei and Fructus Gardeniae decoction, Yinchenhao
Tang are as follows: T1/2 is 0.29, 1.30, 1.07, and 1.75 h, AUCÆt
is 919.1, 1215.0, 2035.3, and 2537.9 ng·h/mL, AUC0Æ is
928.5, 1325.9, 2094.4, and 2612.6 ng·h/mL, respectively.
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