Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 8, September 2007, pp. 537-539
Development of HPTLC–UV Absorption Densitometry
Method for the Analysis of Alprazolam and Sertraline in Combination
and Its Application in the Evaluation of Marketed Preparations
K. Venkateswarlu[1], R.K. Venisetty[2], N.R.
Yellu[2], S. Keshetty[3], and M.G. Pai[1]
[1]Department of Pharmaceutical Analysis, Goa College of Pharmacy,
Panaji, Goa;
[2]Department of Pharmaceutical Analysis, Vaagdevi
College of Pharmacy, Warangal 506 001, India; and
[3]University
College of Pharmaceutical Sciences, Kakatiya University, Warangal
506 009, India
A new simple, sensitive, and reproducible high-performance
thin-layer chromatography method for the estimation of alprazolam
and sertraline in combination is developed using silica gel plates
with fluorescent indicators. The system is equipped with an automated
sample applicator, and the detection was performed at 254 nm
by using UV absorption densitometry. The mobile phase consists
of carbon tetrachloride, methanol, acetone, and ammonia in the
ratio 12:3:5:0.1. The retention factor values for alprazolam
and sertraline are found to be 0.52 and 0.70, respectively. The
limit of detection of alprazolam and sertraline in the mixture
of given proportion is observed to be 0.05 µg/mL and 2.5 µg/mL
and the limit of quantitation is 0.2 µg/mL and 10 µg/mL,
respectively. The method has shown good linearity in the range
of 0.2 µg/mL to 0.65 µg/mL for alprazolam (R2 > 0.9953)
and 10 µg/mL to 32.5 µg/mL for sertraline (R2 > 0.9942).
The intra- and inter-assay (n = 5) variations in the linear range
are less than 4% for alprazolam and 6% for sertraline. Three
pharmaceutical products containing this combination are analyzed
to test the applicability of the new method. The percentage of
alprazolam and sertraline in the tablets studied range from 97.7%
to 102.82% and 96.5% to 99.9%, respectively.
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