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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 8, September 2007, pp. 515-518
Comparison of Two Quantitation Methods in HPLC:
Standardless Versus Calibration with External Standards. Application
to the Analysis of Amino Acids in Fruit Juices
A. Versari, G.P. Parpinello, and A. Fabiani
Dipartimento di Scienze degli Alimenti, Università di
Bologna, P.zza Goidanich 60, Cesena (FC) 47023, Italy
A distinctive method of high-performance liquid
calibration peak-area quantitation, called the standardless or
absolute method, allows the absolute number of moles of the main
amino acids found in twenty-six commercial fruit juices to be
determined. The theory of the standardless model is briefly illustrated.
The results found with this alternative method are compared with
the reference procedure (calibration with external standards)
by using difference plots and regression analysis. The presence
of both systematic, constant, and proportional errors between
the two methods is disclosed and discussed.
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