Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 8, September 2007, pp. 507-514
Improved High-Performance Liquid Chromatography–Diode
Array Detection Method for the Determination of Phenolic Compounds
in Leaves and Peels From Different Apple Varieties
Maddalena Kindt[1], M. Cristina Orsini[2], and
Barbara Costantini[3]
[1]Dipartimento di Scienze Aziendali. Area: Tecnologie e Valorizzazione
delle Risorse. Piazza Scaravilli, 1 40126 Bologna Italy;
[2]Dipartimento
di Colture arboree. Viale Fanin 46 Facoltà di Agraria,
Bologna, Italy; and
[3]Facoltà di Farmacia, Università di
Bologna, Italy
Hydroxycinnamic acids derivatives, monomeric and
oligomeric flavan-3-ols, flavonols, and dihydrocalcones are four
of the major polyphenolic groups found in apples leaves and peels.
A simple extraction with minimal pre-treatment and a high-performance
liquid chromatography–diode array detection determination
are optimized and validated, in order to identify and quantitate
the polyphenolic profile of leaves and peels of four apples varieties
(Gala, Topaz, Golden Delicious, and Florina). The improved chromatographic
method has led to better separation of some known polyphenols
in a single course, and diode-array detection has been used for
the previsional identification of some polyphenolic compounds
not available as standards. Because the mobile phase and the
chromatographic column are compatible with a mass spectrometer,
this method could investigate the unknown flavanols, flavonols,
hydrocinnamic acid derivatives, and chalcone-related compounds
found in apple leaves and peel extracts analyzed.
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