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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 9, October 2007, pp. 629-634
Non-Intuitive Separation of Vanilla Compounds
Using Rapid Resolution High-Performance Liquid Chromatography
Jean-Paul Larcinese, Fabrice Avaltroni, and Valéry
Normand
FIRMENICH SA, P.O. Box 148, CH-1217 MEYRIN 2 (Geneva), Switzerland
A method is presented for modeling the retention
peak migration in rapid resolution high-performance liquid chromatography
(HPLC) depending on experimental parameter values. It allows
time reduction on the determination of the experimental conditions
for optimal resolution (especially for untrained chromatographers).
Separation for 18 species present in a conventional vanilla formulation
was not possible in a single chromatogram, due to a systematic
error in defining single peak migration with the usual assumptions.
This was achieved by the means of two runs under different experimental
conditions. Prediction of the peak inversion for quantitation
purposes in a given mixture is now possible and can help to avoid
misidentification on set-ups with UV–ELSD or other non-specific
detectors.
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