Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 9, October 2007, pp. 610-615
Simultaneous Determination of Active Ingredients
in Ethnomedicine Gaultheria leucocarpa var. yunnanensis and its
Medicinal Preparation by Capillary Electrophoresis with Electrochemical
Detection
Yikun Zeng[1], Zhuxing Tang[1,2], Qingjiang Wang[1],
Pingang He[1], and Yuzhi Fang[1],
[1]Department of Chemistry,
East China Normal University, Shanghai 200062, China and
[2]Department
of Chemistry , Shenyang Ligong University, Shenyang 110168, China
A simple and rapid capillary electrophoresis (CE)
with electrcochemical detection (ED) method has been established
for the simultaneous determination of seven active ingredients
in the stems and roots of Gaultheria leucocarpa var. yunnanensis
and its medicinal preparation, including (+)-catechin, rutin,
gentisic acid, vallinic acid, salicylic acid, quercetin, and
protocatechuic acid. The effects of working potential, pH, and
concentration of running buffer, separation voltage, and injection
time on CE–ED are systematically investigated. Under the
optimum conditions, the seven analytes could be completely separated
within 23 min in a borax running buffer (pH 8.7). A good linear
relationship is obtained over three orders of magnitude with
detection limits (signal-to-noise ratio = 3) ranging from 5 × 10–8
g/mL to 3 × 10–7 g/mL for the analytes. The proposed
method is successfully used in the analysis of real samples after
a relatively simple extraction procedure, and the assay results
are satisfactory.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
