Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 9, October 2007, pp. 605-609
Enantiomer Separation of the Four Diastereomers
of Guaiacyl Glycerol from Hydnocarpus annamensis by Capillary
Electrophoresis with HP-β-CD as a Chiral Selector
Yi Liu[1], Haiming Shi[2], Zhe Sun[1], Xiaomei
Ling[1], and Pengfei Tu[2]
[1]Department of Pharmaceutical Analysis, School of Pharmaceutical
Sciences, Peking University, Beijing 100083, China and
[2]Department
of
Natural Medicines, School of Pharmaceutical Sciences, Peking
University, Beijing 100083, China
A capillary electrophoresis method with HP-ββ-CD
as the chiral selector is established for the enantioseparation
of two pairs of phenylpropanoids, which are isolated from Hydnocarpus
annamensis. The effects of buffer pH, HP-ββ-CD and
buffer concentration, applied voltage, and cartridge temperature
on the enantioseparation are optimized. A baseline separation
of the four diastereomers of guaiacyl glycerol is achieved in
less than 10 min under these optimized conditions: 25 mmol/L
Borax–NaOH buffer (pH 10.01) in the presence of 30 mmol/L
HP-ββ-CD at 15°C and 30 kV. The experimental results
show that the reported method by capillary electrophoresis for
the separation of the four diastereomers of guaiacyl glycerol
is powerful, sensitive, and fast, requires smaller amounts of
reagents, and can be employed as a reliable alternative to other
methods.
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