Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 9, October 2007, pp. 600-604
Separation and Determination of Flavonoids in
Ixeridium gracile by Capillary Electrophoresis
Yu Zhang[1,2], Liang Zhao[1], and Yan-Ping Shi[1],
[1]Key Laboratory for Natural Medicine of Gansu Province, Lanzhou
Institute of Chemical Physics, Chinese Academy of Sciences,
Lanzhou 730000, P.R. China and
[2]Graduate University of Chinese
Academy of Sciences, Beijing 100039, P. R. China
A simple and rapid capillary electrophoresis method
has been developed for the quantitative analysis of three active
compounds: (3R)-7,2'-dihydroxy-3',4'-dimethoxy-isoflavan, kaempferol,
and quercetin in Ixeridium gracile. The buffer solution used
in this method is 20 mmol/L borate at pH 9.5. The effects of
pH value, borate concentration, and applied voltage on migration
behavior are systematically investigated. Regression equations
reveal good linear relationships (correlation coefficients: 0.9986,
0.9997, and 0.9999) between the peak area of each compound and
its concentration. The relative standard deviations of the migration
time and peak area are less 1.12% and 3.11% (intraday), and 1.43%
and 3.52% (interday), respectively, under the optimized separation
conditions. The contents of the three flavonoids in I. gracile
are successfully determined within 7.8 min, with satisfactory
repeatability and recovery.
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