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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 9, October 2007, pp. 593-599
Separation of Chlorins and Carotenoids in Capillary
Electrophoresis
Gamze Kavran Belin and Fazil O. Gülaçar
Laboratory of Mass Spectrometry, University of Geneva, Sciences
I, Boulevard d’Yvoy 16, CH-1211 Geneva 4, Switzerland
The present study reports the investigation of
capillary electrophoresis (CE) for the separation of the photosynthetic
pigments (chlorophyll derivatives as well as carotenoids) together.
Various CE methods, such as micellar electrokinetic chromatography,
capillary electrokinetic chromatography, and nonaqueous capillary
electrophoresis (NACE) are tested, with coated and uncoated capillary
columns to evaluate optimal separation conditions using diode
array detection. The effect of different type and composition
of organic solvents and surfactants on the separation is discussed.
Detection limits are found in the range of 1.14–2.45 ppm.
According to the system suitability results, the most effective
separation is observed using NACE with Aliquat 336 as cationic
surfactant in coated capillary and mixture of MeOH–ACN–THF
(5:4:1, v/v/v) as solvent. Quantitative evolution is investigated,
and recovery percentage values are found to be 96.7–102%.
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