|
Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 9, October 2007, pp. 587-592
Application of Protein–Liposome Conjugate
as a Pseudo-Stationary Phase in Capillary Electrophoresis
Jing Jiang, Lina Geng, Feng Qu, Aiqin Luo,
Hong Li, and Yulin Deng
School of Life Science and Technology, Beijing Institute of Technology,
Beijing 100081, China
Liposomes have very similar structure to cell plasma
membranes. Using liposomes as stationary phase in liquid chromatography
(LC) or micellar electrokinetic chromatography (MEKC) has been
demonstrated to be a good, dynamic method for the study of the
interaction between cell membranes and important biomolecules.
There has been no report on integrating plasma membrane proteins
with phospholipids as pseudo-stationary phase in MEKC. In this
paper, a novel mode of capillary electrophoresis (CE) is developed,
that is, protein–liposome conjugate. This protein–liposome
biomimetic membrane is demonstrated for the first time to be
applicable as pseudo-stationary phase in MEKC. The protein is
able to significantly improve chromatographic performance and
stability. The experimental phenomena are further confirmed in
terms of specific capacity factors and free binding energy. This
new CE mode is used to investigate the interaction between dopamine
transporter and dopamine–nomifensine.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted. To
order electronically
click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers. |
Site Map: Home
| Current Issue | Subscribe
| Back Issues | About
Us | Meetings | Advertising
|
| Books for Sale | For
the Author | Links | Supplier
Info | Search | |
