Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 10, November/December 2007, pp. 706-709
Development and Validation of a TLC-Densitometric
Method for the Simultaneous Quantitation of Strychnine and Brucine
from Strychnos spp. and its Formulations
Kamlesh Dhalwal, Vaibhav M. Shinde, Ajay G.
Namdeo, Kakasaheb R. Mahadik, and Shivajirao. S. Kadam
Poona College of Pharmacy, Bharati Vidyapeeth Deemed University,
Erandwane, Pune- 411 038, Maharashtra, India.
A simple, sensitive, and specific thin-layer chromatography
densitometric method has been developed for the simultaneous
quantitation of strychnine and brucine. These two marker compounds
are quantitated in the seeds of Strychnos nux-vomica, Strychnos
ignatii, and its formulations. The method involves densitometric
evaluation of strychnine and brucine after resolving it by high-performance
TLC on silica gel plate with toluene–ethyl acetate–diethyl
amine–methanol (7:2:1:0.3 v/v) as the mobile phase. The
method is validated for precision (interday and intraday), repeatability,
and accuracy. The relationship between the concentration of standard
solutions and the peak response is linear within the concentration
range of 160 to 480 ng/spot for strychnine and 80 to 480 ng/spot
for brucine. Instrumental precision is found to be 0.54 and 0.78
(% CV), and repeatability of the method is 1.01 and 1.06 (% CV)
for strychnine and brucine, respectively. Accuracy of the method
is checked by recovery study conducted at three different levels
and the average percentage recovery is found to be 99.13% for
strychnine and 100.16% for brucine. The proposed HPTLC method
for the simultaneous quantitation of strychnine and brucine is
found to be simple, precise, specific, sensitive, and accurate,
and it can be used for routine quality control of raw material
of Strychnos spp. It also can be applied in quantitating any
of these marker compounds in other formulations.
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