Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 10, November/December 2007, pp. 701-705
Optimization of a High-Performance Liquid Chromatography
Method for the Separation and Identification of Six Different
Classes of Phenolics
Dhirendra P. Singh[1], R. Govindarajan[1], Anakshi
Khare[2], and Ajay K. S. Rawat[1],
[1]Pharmacognosy and Ethnopharmacology Division, National Botanical
Research Institute, Lucknow – 226 001, India and
[2]Chemistry,
Lucknow University, Lucknow-226 001, India
Flavonoids are chemical moieties widely distributed
in certain plants that are important biologically active constituents
of a daily human diet, with significant pharmacological potential
(anti-hepatotoxic, anti-allergic, anti-inflammatory, anti-osteoporotic,
and anti-tumor activities). Thus keeping in view the importance
of this class of compounds, a rapid method for the separation
and identification of fifteen phenols belonging to six different
types of phenolics in a sole analysis has been developed and
validated using selectivity, precision, recovery, and robustness
as parameters. The method developed, which is rapid, accurate,
and robust for the analysis of different classes of phenols,
can be used in the quality control and standardization of plant
extracts as well as herbal drugs, including compound herbal formulations.
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