Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 45,
Number 5, May/June 2007, pp.286-290
Fluconazol Method Validation by RP-HPLC for Determination
in Biological Skin Matrices
Alessandra C. Ayub[1], Cristina D. Vianna-Soares[2],
and Lucas A.M. Ferreira[2]
[1]Laboratório Globo Ltda., Rod. MG424, km 8.8, São
José da Lapa, 33350-000, MG, Brazil and
[2]Department of Pharmaceutical Products, Federal University of
Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, 31270-901,
MG, Brazil
The bis-triazole antifungal fluconazole (FCZ) is
used in the systemic treatment of superficial mycoses. The inconvenience
of drug interactions and incidence of adverse reactions occurs
in approximately 16% of patients, despite several advantages against
systemic fungal infections. Because its pharmacokinetics profile
is favorable to cutaneous accumulation, it presents a prominent
importance in the treatment of superficial mycoses. This study
shows FCZ method validation by reversed-phase high-performance
liquid chromatography in the linear range of 2 to 32 µg/mL,
which suitable for application in biological matrices after topical
permeation studies. The method is tested in simulated FCZ alcoholic
solution applied to skin extracts after in vitro permeation studies
using Franz cells. Recovery shows good results (in the range 75.0%
± 4.1% to 82.0% ± 6.6%) regarding the biological
matrices.
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