Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 45,
Number 5, May/June 2007, pp.269-272
Determination of Ethambutol Hydrochloride in the
Combination Tablets by Precolumn Derivatization
Ming Yan, Tao Guo, Hongtao Song, Qingchun Zhao,
and Yin Sui
Department of Pharmacy, The Northern Hospital, Wenhua Road 83,
Shenyang, 110016, P.R. China
A new high-performance liquid chromatography method
for the quantitative determination of ethambutol hydrochloride
in combination tablets is presented. Ethambutol is derivatized
with phenylethylisocyanatate at room temperature (22 ±
2°C) for 5 min. Separation is performed by a C18 column using
methanol–water–glacial acetic acid (70:30:0.2, v/v/v)
as the mobile phase. The method is linear for drug concentrations
in the range of 20–120 µg/mL (r = 0.9995). The intra-
and inter-day precisions are lower than 1.46% and 2.22%, respectively.
The average recovery of the samples at three levels is 99.8%.
The results show that derivatization of ethambutol is stable at
30°C for 24 h. This method is simple, rapid, and stable in
the presence of common excipients and antituberculosis drugs in
the tablets.
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