Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 45,
Number 5, May/June 2007, pp.246-250
Chiral Separation of Ephedrine Isomers by Capillary
Electrophoresis Using Bovine Serum Albumin as a Buffer Additive
Nengsheng Ye[1,2], Xuexin Gu[2], and Guoan Luo[1]
[1]Department of Chemistry, Tsinghua University, Beijing 100084,
P.R. China and
[2]Department of Chemistry, Capital Normal University, Beijing
100037, P.R. China.
A method utilizing bovine serum albumin (BSA) as
buffer additive for chiral separation by means of capillary electrophoresis
is described. Parameters that affect chiral separation, such as
buffer pH, buffer concentration, BSA concentration, and organic
modifier, are investigated. Baseline resolution of ephedrine–pseudoephedrine
and norephedrine–norpseudoephedrine isomers are achieved
in an uncoated capillary with a 20 mmol/L phosphate buffer at
pH 9.0 in the presence of 10 µmol/L BSA and 15% (v/v) 2-propanol
at 25°C. The developed method can be applied for the analysis
of ephedra plant extracts that contain the four test drugs.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
