Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 45,
Number 5, May/June 2007, pp.242-245
Development of a Method for the Determination of
Ibafloxacin in Plasma by HPLC with Flourescence Detection and
Its Application to a Pharmacokinetic Study
Pedro Marín[1], Carlos M. Cárceles[1],
Elisa Escudero[1], Ruperto Bermejo[2], and Emilio Fernández-Varón[1],
[1]Department of Pharmacology, Faculty of Veterinary Medicine,
University of Murcia, Campus de Espinardo, 30.071-Murcia, Spain
and
[2]Department of Physical and Analytical Chemistry, University
of Jaen. EUP Linares, 23700, Jaen, Spain
A simple, rapid, and sensitive high-performance
liquid chromatographic method is developed for the determination
of ibafloxacin in rabbit plasma. Plasma proteins are precipitated
with acetonitrile, and after extraction with methylene chloride
followed by desecation, ibafloxacin is determined by reversed-phase
chromatography with fluorescence detection exciting at 330 nm
and emission at 368 nm. Peaks corresponding to ibafloxacin and
the internal standard (salycilic acid) are obtained at 9.8 and
5.2 min, respectively. The method is validated for a limit of
quantitation of 10 ng/mL. The intraday relative standard deviation
ranges from 4.78–7.15%, and the interday precision ranges
from 1.32–4.03%. The method shows linearity for the two
calibration curves used (10–100 ng/mL and 100–2000
ng/mL). The procedure described is applied successfully to a pharmacokinetics
study of ibafloxacin in rabbits.
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