Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 45,
Number 5, May/June 2007, pp.236-241
Rapid Determination of Bisphosphonates by Ion Chromatography
with Indirect UV Detection
Christian Fernandes, Rodrigo S. Leite, and Fernando
M. Lanças
University of São Paulo, Institute of Chemistry at São
Carlos, Laboratory of Chromatography, P.O. Box 780, CEP 13566-590,
São Carlos–SP, Brazil
Rapid methods for etidronate, clodronate, pamidronate,
and alendronate assays are presented. The methods are based on
ion chromatography with indirect UV detection, which avoids the
need for chemical derivatization procedures. Each compound is
analyzed on an individual basis. There is no need for having separation
among these analytes because the aim of the proposed methods is
to analyze each compound separately either in bulk material or
pharmaceuticals. Phenosphere (150 ¥ 2.0-mm, 5 µm) and
Sphereclone (250 ¥ 2.0-mm, 5 µm) anion exchange columns
were employed with sodium citrate (20mM) as the mobile phase.
The methods are simple, rapid (analysis time of 5 min for etidronate
and clodronate and 7 min for alendronate and pamidronate), and
demonstrate precision (relative standard deviation was lower than
2.0% in all concentrations), accuracy, and specificity. Calibration
curves are linear with r2 > 0.99 over the concentration range
of 50 to 400 µg/mL for etidronate and clodronate, and of
100 to 500 µg/mL for pamidronate and alendronate. Furthermore,
they employed silica-based columns, which are cheaper than polymeric
columns frequently used in previous reported methods.
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