Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 6, July 2007, pp.354-359
Separation and Determination of Three Phenylpropanoids
in the Traditional Chinese Medicine and Its Preparations by Capillary
Electrophoresis
Fei Li, Qiu-E Cao, and Zhongtao Ding
Department of Chemistry, Center for Advanced Studies of Medicinal
and Organic Chemistry, Yunnan University, Kunming 650091, P.R.
China
Three phenylpropanoids (ferulic acid, chlorogenic
acid, and caffeic acid) are simultaneously separated and determined
within 13 min by a new capillary electrophoresis method using
15 mmol/L sodium borate (pH 8.71) as run buffer. The optimum conditions
for the separation as well as the analytical characteristics,
such as the calibration graph and limit of detection (LOD) for
the determination of these three compounds, are studied. The linear
range for the determination of ferulic, chlorogenic, and caffeic
acid is 5.0~70.0, 8.0~112.0, and 9.0~64.0 µg/mL, with the
LOD as 1.5, 2.25, and 6.0 µg/mL, respectively. The method,
which is very simple, rapid, and of requisite sensitivity and
reproduction, is satisfactorily used for the separation and determination
not only of ferulic, chlorogenic, and caffeic acid in Cimicifuga
foelida Li and its preparation (Yin-huang-han-pian), but also
of ferulic acid and chlorogenic acid in Ligusticum chuanxiong
hort. and Angelica sinensis (Oliv.) Diels.
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