Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 6, July 2007, pp.350-353
Simultaneous Determination of Four Lignan Compounds
in Herpetospermum caudigerum by Normal-Phase High-Performance
Liquid Chromatography
Hai-Long Yuan[1], Ming Yang[2], Rong-Hui You[2],
Yi Liu[3], Jun Zhu[3], Huan Xie[3], and Xiao-He Xiao[1]
[1]Institute of Chinese Medicine, 302 Hospital of PLA, Beijing
100039;
[2]Key Lab of Modern Preparation of TCM, Ministry of Education,
Nanchang 330004; and
[3]Acadamy of Pharmacy, Chengdu University of TCM, Chengdu 610075,
China
A normal-phase high-performance liquid chromatographic
method with diode array UV detection is developed for the simultaneous
quantitation of four lignan compounds in Herpetospermum caudigerum.
This analysis provides a good resolution and reproducibility.
Chromatography is carried out with a mobile phase of N-hexane–dichlormethane–methanol
(42.5:42.5:5, v/v) at a flow rate of 1.0 mL/min. UV detection
is performed at 280 nm. The calibration curve for lignans concentration
is linear over the range of 2.10 to 42.0 µg/mL, 15.26 to
305.2 µg/mL, 6.15 to 123.0 µg/mL, and 6.24 to 124.8
µg/mL, respectively. The limit of quantitation and detection
for compounds 1, 2, 3, and 4 is 1.31, 2.74, 2.63, and 2.17 µg/mL
and 0.28, 0.25, 0.27, and 0.31 µg/mL, respectively. The
validation data show that the assay is sensitive, specific, accurate,
and reproducible for the simultaneous quantitation of four compounds.
This rapid method is therefore appropriate to quantitate these
lignans in Herpetospermum caudigerum.
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