Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 6, July 2007, pp.325-329
Determination of ng Rivanol in Human Plasma by
SPE-HPLC Method
Zhiyong Guo, DanyiWei, Ning Gan, Hongzhen Xie,
and Xufei Yu
Faculty of Materials Science and Chemical Engineering, The State
Key Laboratory Base of Novel Functional Materials and Preparation
Science, Ningbo University, 315211 Ningbo, China
A high-performance liquid chromatography assay is
described for the determination of rivanol in human plasma. Solid-phase
extraction cartridges are used to extract plasma samples. Separation
is done by using a C18 column. The mobile phase is a mixture of
methanol–0.05% sodium dodecylsulfonate (70:30, v/v, pH 3),
with the flow rate at 1.0 mL/min. UV detection of rivanol is at
272 nm. The calibration curve is linear in the concentration range
of 1 * 10–8 mol/L to 1 * 10–5 mol/L with linear correlation
coefficient r equal to 0.9998. The limit of detection for the
assay is 3 * 10–9 mol/L, corresponding to 1.1 ng/mL. Precision,
expressed as the within- and between-day coefficient of variation,
is 3.3–8.1% and 4.1–9.5%, respectively, at plasma
control samples of 5 * 10–8, 5 * 10–7, and 5 * 10–6
mol/L. And the recovery ranges from 94.8% to 107.2%. The selectivity
of the method is confirmed. Plasma samples are stable for at least
15 days if they are stored lightproof at –20°C. This
method is simple, sensitive, and accurate, and it allows for the
determination ng rivanol in human plasma. It could be applied
to assessing its plasma level in women receiving an intra-amniotic
injection of rivanol.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00.
Visa, MasterCard & Amex accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
