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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 6, July 2007, pp.298-304
Evaluation of Ultra-Performance Liquid Chromatography
in the Bioanalysis of Small Molecule Drug Candidates in Plasma
Lee Goodwin, Stephen A.White, and Neil Spooner
Worldwide Bioanalysis, Glaxosmithkline Research and Development,
Park Road,Ware, Hertfordshire, SG12 0DP, UK
Ultra-performance liquid chromatography (UPLC) combined
with
mass spectrometric detection (MS) is used successfully in the
bioanalysis of small molecule drug candidates in plasma. UPLC–MS
is shown to increase sample throughput by reducing run times
over 3-fold, without compromising analytical sensitivity or analyte
resolution. The technique is demonstrated to be practical and
robust on a commercially available ultra-high pressure system
when injecting extracts of plasma and has also shown to be a
technique that can be used effectively on a conventional highperformance
liquid chromatography system fitted with
short columns (≤ 30 mm in length).
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