Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 45,
Number 2, February 2007, pp.70-75
Liquid Chromatographic Analysis of Glucosamine
in Commercial Dietary Supplements Using Indirect Fluorescence
Detection
Xiaoxuan Shen[1], Min Yang[2], and Sterling A.
Tomellini[1],
[1]Department of Chemistry, University of New Hampshire, Durham,
NH 03824 and
[2]Novartis Pharmaceuticals Corporation, One Health Plaza, East
Hanover, NJ 07936
A method of using indirect fluorescence detection
is evaluated for the analysis of glucosamine in commercial dietary
supplements following chromatographic separation. In this method,
the eluting analyte, glucosamine, was detected by monitoring an
increase in the fluorescence signal for l-tryptophan (L-Trp) or
dl-5-methoxytryptophan (5-MTP) after glucosamine complexed with
a copper(II) ion and released either L-Trp or 5-MTP from a copper(II)
complex, which is introduced postcolumn. The fluorescence of L-Trp
and 5-MTP are quenched when complexed with the copper(II) ion.
The results obtained using indirect fluorescence detection are
compared with the results obtained for precolumn derivatization
with phenylisothiocyanate. Statistical analysis is performed to
compare the results obtained for the two postcolumn interaction
components, Cu(L-Trp)2 and Cu(5-MTP)2, as well as the results
obtained using the indirect fluorescence detection method and
a precolumn derivatization method. The indirect fluorescence detection
method provided an alternative to precolumn derivatization for
determining the concentration of glucosamine in commercial dietary
supplements. The stability of the glucosamine–o-phthalaldehyde–3-mercaptopropionic
acid derivative is also evaluated to investigate the applicability
of the popular precolumn derivatization reagent, o-phthalaldehyde–3-mercaptopropionic
acid, for this analysis.
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