Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 45,
Number 4, April 2007, pp.216-219
Simultaneous Determination of Seven Flavonoids
in Potentilla multifida by HPLC
Peifeng Xue[1], Yuying Zhao[1,2], Bin Wang[1],
and Hong Liang[1],
[1]Department of Natural Medicines and
[2]State Key Laboratory of Natural and Biomimetic Drugs, School
of Pharmaceutical Sciences, Peking University Health Science Center,
Beijing 100083, P.R. China
A reversed-phase high-performance liquid chromatographic
method is described for the simultaneous determination of seven
flavonoids in Potentilla multifida: hyperin, quercetin-3-O-b-d-glucopyranoside,
luteolin-7-O-b-d-glucuronide, apigenin-7-O-b-d-glucuronide, quercetin,
tribuloside, and apigenin. The method involves the use of a Hypersil
octadecylsilyl silica (ODS) analytical column (125Å, 5 µm,
4.6 ¥ 250 mm) at 25°C with the mixture of acetonitrile
and aqueous H3PO4 as the mobile phase and detection at 254 nm.
The recovery of the method is 95.4–104.8%, and linearity
(r > 0.9998) is obtained for all the flavonoids. The results
indicate that the flavonoid content of P. multifida varied significantly
from locality to locality.
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