|
Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 45,
Number 4, April 2007, pp.207-211
Retention of Histidine-Containing Peptides on a
Nickel Affinity Column
Igor A. Kozlov, Bahram G. Kermani, Peter C. Melnyk,
David L. Barker, Chanfeng Zhao, John P. Hachmann, and Michal Lebl
Illumina, Inc., 9885 Towne Centre Drive, San Diego, CA 92121
The retention of histidine-containing peptides in
immobilized metal-affinity chromatography is studied using several
hundred modeled peptides. Retention is driven primarily by the
number of histidine residues; however, the amino acid composition
in the immediate vicinity plays a significant role. Specifically,
the arginine and tryptophan content has to be taken into consideration.
During the course of this study, an alternative tag that can be
used similarly to a polyhistidine tag is discovered.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted. To
order electronically
click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers. |
Site Map: Home
| Current Issue | Subscribe
| Back Issues | About
Us | Meetings | Advertising
|
| Books for Sale | For
the Author | Links | Supplier
Info | Search | |
