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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 45,
Number 4, April 2007, pp.189-194
Triterpenoids From Swallow Roots— A Convenient
HPLC Method for Separation
S. Nagarajan and L. Jagan Mohan Rao
Plantation Products, Spices and Flavor Technology Department,
Central Food Technological Research Institute, Mysore–570
020, India
A convenient semi-preparative high-performance liquid
chromatography (HPLC) method for separating a mixture of triterpenoids
(a-amyrin, b-amyrin, and lupeol) and their corresponding acetates
from the swallow roots (Decalepis hamiltonii Wight and Arn), which
are known to have potential bioactive properties, is described.
The swallow roots are found to be one of the richest natural sources
for these compounds. The hexane extract of the dried spent root
on column chromatography yields mixtures (i.e., triterpenoids
and their acetates) containing at least three compounds in each.
These could not be further separated using the routine chromatographic
techniques, such as classical column chromatography and preparative
thin-layer chromatography using various solvent systems. Therefore,
the optimal conditions are determined on reversed-phase HPLC for
their separation and are characterized using spectral data, particularly
by nuclear magnetic resonance with physical and chemical properties.
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