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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 45,
Number 1, January 2007, pp.28-32
Analysis of Micafungin in Serum by Capillary Zone
Electrophoresis
Toshihiro Kitahashi and Itaru Furuta
Department of Laboratory Medicine, Kinki University School of
Medicine, 377-2 Ohno-higashi, Osakasayama, Osaka 589-8511, Japan
A method is developed for measuring the concentration
of micafungin, an echinocandin antifungal agent, in serum, using
capillary zone electrophoresis. With a 0.1M borate buffer (pH
10.0) as the run buffer, detection is carried out at 200 nm. Pretreatment
is performed by adding acetonitrile to serum (1:2) for deproteinization,
allowing the supernatant fluid to be taken for measurement. The
detection limit of the assay is 0.5 mg/L at a signal-to-noise
ratio of 3.0. Coefficients of variation for intra- and inter-assay
precision are 3.45–4.47% and 2.61–7.15%, respectively,
at a nominal concentration of 5.0–25.0 mg/L. Their recovery
rates are 92–110%. It is convenient for the monitoring of
micafungin therapy in patients contracting clinically important
deep mycoses.
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