Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 7, August 2007, pp. 447-454
?Thin-Layer Chromatography of Alkaloids on Cyanopropyl
Bonded Stationary Phases. Part I.
?Anna Petruczynik[1], Monika Waksmundzka-Hajnos[1],
Tomasz Michniowski[1], Tomasz Plech[2], Tomasz Tuzimski[3], Michal
L. Hajnos[4], Maria Gadzikowska[1], and Grzegorz Józwiak[1]
[1]Departament of Inorganic Chemistry, Faculty of Pharmacy, Medical
University of Lublin, Staszica 6, 20-081 Lublin, Poland;
[2]Students’ Scientific
Association at the Department of Inorganic Chemistry, Medical
University of Lublin, Lublin, Poland;
[3]Departament of Physical
Chemistry, Faculty of Pharmacy, Medical University of Lublin,
Staszica 6, 20-081 Lublin, Poland; and
[4]Departament of Pharmacognosy,
Faculty of Pharmacy, Medical University of Lublin, Chodzki 1,
20-093 Lublin, Poland
?Selected alkaloids are chromatographed on cyanopropyl–silica
thin layers using various nonaqueous and aqueous eluents. Because
of the strong retention of these basic compounds, nonaqueous
eluents containing medium polar diluents, strongly polar modifiers,
and silanol blockers (ammonia or diethylamine) are required for
separation. Likewise, aqueous eluents containing modifiers (acetonitrile,
methanol, and tetrahydrofuran), buffered aqueous solutions at
pH 2–8, ion-pair reagents [octane sulfonic acid sodium
salt, sodium dodecyl sulphate, and bis-(2-ethylhexyl)phosphoric
acid], or silanol blockers (ammonia, tetrabutyl ammonium chloride,
and diethyl amine) are investigated. The separation selectivity
as well as spot symmetry and efficiency system in the applied
eluent systems are analyzed. The most selective and efficient
systems are used in two-dimensional separations of isoquinoline
alkaloids’ mixture and the plant extracts Chelidonium majus,
Fumaria officinalis, and Glaucium flavum. Two-dimensional thin-layer
chromatography on cyanopropyl layer with diode array detection
densitometry enables the separation and identification of some
alkaloids in plant extracts.
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