Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 7, August 2007, pp. 435-438
?Biological and Chemical Detections in Adsorbent
Layer for Monitoring Microbial Production of Primycin
?Antal Szabó, Attila Kónya, Valéria
Széll, György Máté, and Balázs
Erdélyi
IVAX Drug Research Institute Ltd., Berlini u. 47-49, 1049 Budapest,
Hungary
?Primycin is a macrolide antibiotic complex produced
in microbiological fermentation processes. The microbial production
of primycin requires an in-process analytical method suitable
for monitoring the level of the active agents. In this paper,
a method fulfilling the described requirement is presented. This
method consists of a simple, efficacious extraction step, an
instrumental sample application folowed by a high-performance
thin-layer chromatographic separation in relatively short time
and a quantitative chromatogram evaluation. A dipping technique,
in a solution containing sulfuric acid followed by heating at
120°C, is used for chromogen formation, resulting in an absorption
maximum at 290 nm. A progress diagram of the fermentation obtained
by this technique is compared with one obtained by a microbiological
agar diffusion method. The bioautographic evaluation of the active
spots in the chromatogram are also presented. By our TLC method,
the group of the active primycin components in the fermentation
broth and by-products formed during the fermentation can be well
separated. The relative intensities of the different TLC spots
provide some information on the formation of the active components.
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