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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 7, August 2007, pp. 422-427
?Development of a Gas Chromatographic Test for
the Quantitation of the Biomarker 2-Butoxyacetic Acid in Urine
Samples
?C. B’Hymer
U.S. Department of Health and Human Services, Centers for Disease
Control and Prevention, National Institute for Occupational
Safety and Health, Taft Laboratory, 4676 Columbia Parkway,
Cincinnati, OH 45226
?An accurate and precise method is developed and
evaluated for the detection and quantitation of 2-butoxyacetic
acid (2-BAA), a metabolite and biomarker for human exposure to
2-butoxyethanol. The solvent 2-butoxyethanol (2-BE) is extensively
used in various industrial and domestic applications, and it
is a health concern owing to its toxicity. Sample preparation
consists of liquid–liquid extraction (LLE) of urine, then
esterification of 2-BAA to produce the ethyl ester analog. The
gas chromatographic conditions utilize a dimethyl polysiloxane
phase (HP-1) capillary column and a mass spectrometer (MS) for
detection of the analyte. Validation of this method includes
a recovery study using fortified urine samples, which demonstrated
good accuracy and precision; recovery varied between 100% and
102% of theory, with relative standard deviations of replicate
samples at 2.8% and less. The detection limit of this method
ranges from 0.005 to 0.015 µg/mL equivalent level of 2-BAA
in urine.
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