Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 7, August 2007, pp. 409-414
??Development and Validation of a Simple and Rapid
HPLC Method for the Quantitative Determination of Voriconazole
in Rat and Beagle Dog Plasma
?Shan Cheng[1], Feng Qiu[2], Jia Huang[3], and
Junqi He[1]
[1]Department of Biochemistry and Molecular Biology, School of
Basic Medical Sciences, Capital Medical University, No.10 Xitoutiao,
You An Men, Beijing 100069, China;
[2]College of Chemistry and
Molecular Engineering, Peking University, No. 202 Cheng Fu Road,
Beijing 100871, China; and
[3]Beijing Tiantan Hospital Affiliated
to Capital Medical University, No. 6 Tiantanxili, Beijing 100050,
China
?A simple and rapid high-performance liquid chromatographic
method with UV detection is developed and validated to determine
the concentration of voriconazole in rat and beagle dog plasma.
After protein precipitation using acetonitrile, the supernatant
solution is chromatographed on a Diamonsil C18 column (250 × 4.6-mm
i.d., 5 µm). The mobile phase used is a combination of
acetonitrile–water–acetic acid (55:45:0.25, v/v/v)
with a pH of 4.0. Detection is achieved by a UV detector monitored
at a wavelength of 256 nm. The matrix calibration curves are
obtained both in the concentration range of 0.10–50.0 µg/mL
in rat and beagle dog plasma, with the lower limit of quantitation
of 0.10 µg/mL. The intra- and inter-assay precisions in
terms of % relative standard deviation are lower than 8.6% and
6.0% in rat and beagle dog plasma, respectively. The accuracy
in terms of % relative error ranged from –0.5% to 8.0%
and –0.5% to 6.0% in rat and beagle dog plasma, respectively.
This validated method is successfully applied to determine the
concentration of voriconazole in plasma after intravenous administration
of 36 mg/kg voriconazole to rats and 10 mg/kg voriconazole to
beagle dogs, respectively.
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