Published:Journal of Chromatographic Science, ISSN 0021-9665 Volume 45, Number 7, August 2007, pp. 405-408

?Determination of Sodium Monofluoroacetate (1080) in Biological Samples as its 4-Bromomethyl-7-Methoxycoumarin Derivative by RP-HPLC

?Zhenming Xie[1], Wenbing Shi[2], Lan Liu[1], and Qinying Deng[1]
[1]School of Chemistry and Chemical Engineering, Sun Yet-Sen University, Guangzhou 510275, PR China and
[2]Public Security Bureau of Guangdong Province, Guangzhou 510050, PR China

?A high-performance liquid chromatography (HPLC) method with fluorescence detection is described for the determination of sodium monofluoroacetate (MFA-Na) in biological samples. 4-Bromomethyl-7-methoxycoumarin is used as a derivatization reagent and reacted with MFA-Na to form 7-methoxy-4-methylenecoumarin monofluoroacetate for HPLC analysis. Chromatographic separation is performed on a Hewlett Packard RP-18 column using methanol–water (60:40, v/v) as the mobile phase. A fluorescent detector is employed with the excitation and emission wavelengths as 319 nm and 390 nm, respectively. The novel method yields a good linear relationship when the concentration of MFA-Na is within 1 and 500 nmol/mL (r = 0.9996). The detection limit is 50 pmol/mL. The established method is applied to determine MFA-Na in biological samples. The recovery rates of MFA-Na are between 81% and 88%, and the relative standard deviations are less than 5%. The method shows good sensitivity and selectivity for the determination of MFA-Na in biological samples.

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