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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
45, Number 7, August 2007, pp. 405-408
?Determination of Sodium Monofluoroacetate (1080)
in Biological Samples as its 4-Bromomethyl-7-Methoxycoumarin
Derivative by RP-HPLC
?Zhenming Xie[1], Wenbing Shi[2], Lan Liu[1],
and Qinying Deng[1]
[1]School of Chemistry and Chemical Engineering, Sun Yet-Sen University, Guangzhou
510275, PR China and
[2]Public Security Bureau of Guangdong Province, Guangzhou 510050, PR China
?A high-performance liquid chromatography (HPLC)
method with fluorescence detection is described for the determination
of sodium monofluoroacetate (MFA-Na) in biological samples. 4-Bromomethyl-7-methoxycoumarin
is used as a derivatization reagent and reacted with MFA-Na to
form 7-methoxy-4-methylenecoumarin monofluoroacetate for HPLC
analysis. Chromatographic separation is performed on a Hewlett
Packard RP-18 column using methanol–water (60:40, v/v)
as the mobile phase. A fluorescent detector is employed with
the excitation and emission wavelengths as 319 nm and 390 nm,
respectively. The novel method yields a good linear relationship
when the concentration of MFA-Na is within 1 and 500 nmol/mL
(r = 0.9996). The detection limit is 50 pmol/mL. The established
method is applied to determine MFA-Na in biological samples.
The recovery rates of MFA-Na are between 81% and 88%, and the
relative standard deviations are less than 5%. The method shows
good sensitivity and selectivity for the determination of MFA-Na
in biological samples.
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