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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 44,
Number 8, September 2006, pp. 523-527
Subcritical Solvent Regeneration of Contaminated
HPLC Columns Used for Separating Biomolecules
Jianmin Zhang and Kevin B. Thurbide
Department of Chemistry, University of Calgary, 2500 University
Drive, N.W. Calgary, Alberta, Canada T2N 1N4
The regeneration of contaminated chromatography
columns using subcritical mobile phases is presented. The method
employs minimal amounts of organic solvents (pressurized and heated
above their boiling point) to clean strongly retained sample components
from hydrophobic stationary phase particles. The process lasts
approximately 1 h and is demonstrated to restore the performance
of polymeric and bonded-phase columns. The technique is simple
to apply and widely accessible, requiring only common laboratory
equipment. Results indicate that this could be a beneficial alternative
approach for cleaning contaminated columns either directly or
in cases in which conventional methods have been unsuccessful.
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