Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 44,
Number 8, September 2006, pp. 518-522
Multiresidue Analysis of 18 Organochlorine Pesticides
in Traditional Chinese Medicine
Lili Hao and Jian Xue
Institute of Medicinal Plant Development, Chinese Academy of Medical
Science and Peking Union Medical College, Beijing 100094, China
A simple and efficient method for simultaneous gas
chromatographic (GC) determination of 18 organochlorine pesticides
[tecnazene, hexachlorobenzene, a-benzenehexachloride (BHC), pentachloronitrobenzene
(PCNB), g-BHC, heptachlor, aldrin, methyl pentachlorophenyl sulfide,
b-BHC, d-BHC, heptachlor epoxide, a-endosulfan, trans-chlorodane,
cis-chlorodane, p,p'-dichlorodiphenyl dichloroethylene, o,p'-DDT,
p,p'-DDD, and p,p'-DDT] in Traditional Chinese Medicine (TCM)
is described. The procedure involves ultrasonic extraction and
sulphuric acid treatment as the cleanup method. Detection of the
sample is performed by GC–electron capture detection. A
series of experiments are conducted to optimize the final pretreatment
conditions [acetone–petroleum ether (1:1) as the extract
solvent, ultrasonication for 15 min, three steps, concentrated
sulphuric acid with 10% water for sulphuric acid treatment]. Recovery
studies are performed at 10, 50, and 100 parts-per-billion (ppb)
fortification levels of each organochlorine pesticide, except
for a-endosulfan, which are 20, 100, and 200 ppb. The percentage
recoveries range from 77.9% ± 6.4% to 114.0% ± 8.1%
(average ± standard deviation). The simple and rapid method
may be used to routinely determine organochlorine pesticides in
TCM.
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