Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 44,
Number 8, September 2006, pp. 510-517
Two-Dimensional Thin-Layer Chromatography of Selected
Coumarins
Monika Waksmundzka-Hajnos[1], Anna Petruczynik[1],
Michal lL. Hajnos[2], Tomasz Tuzimski[3], Anna Hawryl[1], and
Anna Bogucka-Kocka[4]
[1]Department of Inorganic Chemistry, Medical University, Staszica
6, 20-081;
[2]Department of Pharmacognosy, Medical University, Chodzki 1,
20-093;
[3]Department of Physical Chemistry, Medical University, Staszica
6, 20-081; and
[4]Department of Pharmaceutical Botany, Medical University, Chodzki
1, 20-093 Lublin, Poland
Polar-bonded stationary phases (CN-silica and Diol-silica)
are used with nonaqueous eluents in adsorption mode or with aqueous
eluents in partition mode. This enables the application of these
systems in two-dimensional separations because of the different
selectivity and application to the separation of closely related
compounds of similar physicochemical properties and retention
behaviour. Similarly, multiphase plates, connected with C18 strips
and silica layers, are used with aqueous and nonaqueous eluents.
Such layers were applied for the separation of selected coumarins.
Thus, differences in separation selectivity are applied for the
separation of coumarin fractions from plant extracts of the Apiaceae
family by two-dimensional thin-layer chromatography.
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