Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 44,
Number 8, September 2006, pp. 473-478
Simultaneous Determination of Urinary Metabolites
of Methoxypsoralens in Human and Umbelliferae Medicines by High-Performance
Liquid Chromatography
Lai-Hao Wang and Si-Yun Jiang
Department of Applied Chemistry, Chia Nan University of Pharmacy
and Science, Tainan, Taiwan 71710, China
A high-performance liquid chromatography method
has been developed for the determination of coumarins and furocoumarins
(psoralens). Nine coumarins and furocoumarins are separated simultaneously
on a Hypersil C8 (25 cm ¥ 4.6-mm i.d.) column with a gradient
of methanol and acetonitrile aqueous solution as mobile phase
at 1.0 mL/min with two-channel UV–vis absorbance detection.
The limits of detection are 0.366, 0.219, 0.317, 0.440, 0.536,
0.300, 0.531, 0.531, 0.237, and 0.280 ng/mL for coumarin, 7-hydroxycoumarin,
7-methoxycoumarin, citropten (5,7-dimethoxycoumarin), 7-ethoxy-4-methylcoumarin,
psoralen, xanthotoxin (8-methoxypsoralen), bergapten (5-methoxypsoralen),
isopimpinellin (5,8-dimethoxypsoralen), and imperatorin (9-isopenteneoxypsoralen),
respectively. Human urine is analyzed 1–6 days after ingestion
of the oral Chinese medicines. This lead to the conclusion that
the concentration of coumarins and furocoumarins is higher than
that of the control urine. The coumarins and furocoumarins are
detected at 312 and 249 nm, respectively.
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