Published:Journal of Chromatographic Science, ISSN 0021-9665 Volume 44, Number 9, October 2006, pp.561-565

Paola Dugo[1], Maria del Mar Ramírez Fernández[2,3], Antonella Cotroneo[3], Giovanni Dugo[3], and Luigi Mondello[3]
[1]Dipartimento di Chimica Organica e Biologica, Facolta di Science, Università di Messina, Salita Sperone 31, 98166 Messina, Italy;
[2]National Institute of Criminalistic and Criminology, Vilvoordsesteenweg 100, 1120 Brussels, Belgium; and
[3]Dipartimento Farmaco-Chimico, Facolta di Farmacia, Università di Messina, viale Annunziata, 98168 Messina, Italy

The present investigation is based on the evaluation of the performance of a comprehensive two-dimensional liquid chromatography (LC¥LC) system during method optimization. The LC¥LC set-up, operated in normal phase (NP) mode (adsorption) in the first dimension (1D) and reversed-phase (RP) mode in the second dimension (2D), is equipped with a 1D microbore silica column and a 2D monolithic C18 column with a 10-port two position valve as the interface. A photodiode array detector is used after the 2D separation. A possible cause of peak distorsion because of the immiscibility of the mobile phases employed in the two dimensions is resolved. The optimization of the analytical run time and flow rate for both dimensions and the initial gradient in the 2D is carried out with various standard compounds. The potential and versatility of this LC¥LC approach is demonstrated through the separation of 11 standard components, most of them allergens. The latter, which are characterized by a scattered distribution on the 2D space plane, underwent separation on both a hydrophobicity and polarity basis.

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