Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
44, Number 9, October 2006, pp.552-556
HPLC–MS
Analysis of the Riboflavin Crude Product of Semisynthesis
Jianyu Guo[1], Yan Lu[2], and Haiou Dong[3]
[1]Department of Chemistry, Shanghai Normal University, Shanghai 200234;
[2]Department of Chemical Engineering, Shanghai Institute of Technology, Shanghai
200235; and
[3]Tianjin Tasly Pharmaceutical Co., Ltd., Tianjin 300402, China
An accurate and simple analysis method by
high-performance liquid chromatography–mass spectrometry
for the riboflavin crude product of semisynthesis is established
in this study. In this method, a C18 column is used, and the
mobile phase is methanol–water. Under the optimal analysis
conditions established in this study, all components in the
riboflavin crude product are well separated and identified,
respectively. The external standard method is used for the
quantitative analysis, and the results are proven to be accurate.
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