Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume 44,
Number 9, October 2006, pp.548-551
Liquid Chromatographic
Method for the Simultaneous Determination of Imipenem and Sulbactam
in Mouse Plasma
Irene Aparicio, Miguel Angel Bello, Manuel Callejón,
and Juan Carlos Jiménez
Department of Analytical Chemistry, University of Seville, 41011, Seville, Spain
The first analytical method is developed
and validated for the simultaneous determination of imipenem
and sulbactam in mouse plasma. Sample treatment is based on
plasma stabilization with 4-(2-hydroxyethyl)piperazine-ethanesulfonic
acid (HEPES) (0.5 mol/L; pH 7.0)–water–ethylene
glycol (2:1:1, v/v/v), precipitation of plasma proteins with
acetonitrile, centrifugation, evaporation, and reconstitution
with borate buffer. Analytical determination is carried out
by high-performance liquid chromatography with diode-array
detection. Chromatographic separation is achieved within 11
min on a C18 column by gradient elution with borate buffer
(0.1 mol/L, pH 7.2) and methanol. Imipenem and sulbactam are
monitored at 295 and 230 nm, respectively. The overall interday
accuracy is in the range of 95% to 100% and from 98% and 101%
for imipenem and sulbactam, respectively. Interday precision
is below 8% and 4% for imipenem and sulbactam, respectively.
Limits of quantitation of imipenem and sulbactam are 0.05 and
1.0 µg/mL, respectively. The mean extraction recoveries
are 94.5% and 94.2% for imipenem and sulbactam, respectively.
The described method allows an accurate, simple, and rapid
identification and quantitation of imipenem and sulbactam in
mouse plasma. This method is applied to the analysis of imipenem
and sulbactam in mouse plasma after drug administration.
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